李以仁
姓名 李以仁
電子郵件
聯絡電話 02-77496115
網站 https://irenlee.wixsite.com/singlemolecule
實驗室電話 02-77496206
職稱 副教授
研究領域 物理化學,生物物理化學,反應動力學與動態學
學術專長 使用單分子螢光共振能量轉移(Single Molecule Fluorescence Resonance Energy Transfer, smFRET)顯微術,研究生物分子例如DNA與蛋白質的作用機制,並輔以光鉗(Optical Tweezers)研究生物分子之物理化學特性
實驗室 單分子生物物理化學實驗室
  1. I-R. Lee, T. Akinyi, A. G. Kozlov, T. M. Lohman, T. Ha, “Single Molecule Observation of Direct Transfer of Escherichia Coli Single-Strand Binding Protein (SSB) between Single-Stranded DNA Molecules” In Preparation.
  2. I-R. Lee; A. Gahlmann; A. H. Zewail “Structural Dynamics of Free Amino Acids in Diffraction” Angewandte Chemie International Edition, 2012, 51, 99-102. 
  3. A. Gahlmann; I-R. Lee; A.H. Zewail “Direct Structural Determination of Conformations of Photoswitchable Molecules by Laser Desorption-Electron Diffraction” Angewandte Chemie International Edition 2010, 49, 6524-6527. 
  4. I-R. Lee; W. Lee; A. H. Zewail “Dynamics of Electrons in Ammonia Cages: The Discovery System of Solvation” Chemphyschem 2008, 9, 83-88.  
  5. I-R. Lee; L. Baares; A. H. Zewail “Direct Observation of the Primary Bond-twisting Dynamics of Stilbene Anion Radical” Journal of the American. Chemical. Society 2008, 130, 6708-6709. 
  6. H. Y. Chen; I-R. Lee; P. Y. Cheng ”Gas-phase Femtosecond Transient Absorption Spectroscopy” Review of Scientific. Instruments 2006, 77, 076105.  
  7. I-R. Lee; W. Lee; A. H. Zewail “The Primary Step of the Photoactive Yellow Protein: Isolated Chromophore Dynamics and Protein Directed Function” Proceedings of the National Academy of Sciences of the United States of America 2006, 103, 258-262. 
  8. D. H. Paik; I-R. Lee; D. S. Yang; J. S. Baskin; A. H. Zewail “Electrons in Finite-sized Water Cavities: Hydration Dynamics Observed in Real Time” Science 2004, 306, 672-675. 
  9. D. H. Paik; D. S. Yang; I-R. Lee; A. H. Zewail “The Transition State of Thermal Organic Reactions: Direct Observation in Real Time” Angewandte Chemie International Edition 2004, 43, 2830-2834.
  10. I-R. Lee; Y. C. Chung; W. K. Chen; X. P. Hong; P. Y. Cheng “Femtosecond Probing of Photodissociation Dynamics in Acyl Cyanides” Journal of Chemical Physics 2001, 115, 10656-10670.
  11. I-R. Lee; W. K. Chen; Y. C. Chung; P. Y. Cheng “A Direct Observation of Non-RRKM Behavior in Femtosecond Photophysically Activated Reactions” Journal of Physical Chemistry 2000, 104, 10595-10599.
  12. C. G. Lu; R. M. R. Hsieh; I-R. Lee; P. Y. Cheng “Ultrafast Dynamics of Gas-phase Excited-state Intramolecular Proton Transfer in 1-Hydroxy-2-Acetonaphthone” Chemical Physics Letter 1999, 310,103-110. 
年度 論文名稱
2019 Mononuclear Manganese(III) Superoxo Complexes: Synthesis, Characterization, and Reactivity, Inorganic Chemistry, vol. 58, Jul. 2019
2019 Long-Range Hairpin Slippage Reconfiguration Dynamics in Trinucleotide Repeat Sequences, Journal of Physical Chemistry Letters, vol. 10, Jun. 2019
2018 Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device, ACS Sensors, vol. 3, Mar. 2018
2017 Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion, Proceedings of the National Academy of Sciences of the United States of America, vol. 114, Jul. 2017
I-R. Lee, T. Akinyi, T. Ha, “Single Molecule Observation of Direct Transfer of Escherichia Coli Single-Strand Binding Protein (SSB) between Single-Stranded DNA Molecules” Biophysical Society 57th Annual Meeting, February, 2013, Philadelphia, PA, USA. 
年度 論文名稱
2019 Long-Range Slippery Hairpin Reconfiguration and its Mechanism in Trinucleotide Repeats Revealed by Single-Molecule Spectroscopy, Annual Meeting of the Biophysical Society, Mar. 02-06, 2019, Baltimore, USA
2015 The effect of magnesium cations on the folding of Gquadruplex, The 9th International Conference on Advanced Materials and Devices, Dec. 07-09, 2015, Jeju, Korea
2014 Single-molecule study on the formation and dynamics of G-quadruplex in the presence of magnesium cations, 19th East Asian Workshop on Chemical Dynamics, Oct. 04-07, 2014, Gold Coast, Australia
年度 計畫名稱 職稱/擔任之工作 計畫期間 補助/委託或合作機構
2018 單分子結構動態學研究-導致基因疾病的連續核酸序列 主持人 2016.04 ~ 2018.07 科技部(原國科會)
2017 以單分子光譜研究去氧核醣核酸分子之結構動態學 主持人 2014.08 ~ 2017.07 科技部(原國科會)
國家 學校名稱 系所 學位 期間
美國 加州理工學院(Caltech) 化學系 博士 2003.06 ~ 2010.12
台灣 國立清華大學 化學所 碩士 2000.09 ~ 2002.06
台灣 國立清華大學 化學系 理學士 1995.09 ~ 1998.06
台灣 國立交通大學 應用化學系 1994.09 ~ 1995.06
服務機關名稱 單位 職務 期間
美國伊利諾大學香檳分校(UIUC) 物理系 博士後研究員 2012.02 ~ 2014.01
美國加州理工學院(Caltech) 化學系 博士後研究員 2011.01 ~ 2012.01