李以仁
姓名 李以仁
电子邮件
联络电话 02-77496115
网站 https://irenlee.wixsite.com/singlemolecule
实验室电话 02-77496206
职称 副教授
研究领域 物理化学,生物物理化学,反应动力学与动态学
学术专长 使用单分子萤光共振能量转移(Single Molecule Fluorescence Resonance Energy Transfer, smFRET)显微术,研究生物分子例如DNA与蛋白质的作用机制,并辅以光钳(Optical Tweezers)研究生物分子之物理化学特性
实验室 单分子生物物理化学实验室
  1. I-R. Lee, T. Akinyi, A. G. Kozlov, T. M. Lohman, T. Ha, “Single Molecule Observation of Direct Transfer of Escherichia Coli Single-Strand Binding Protein (SSB) between Single-Stranded DNA Molecules” In Preparation.
  2. I-R. Lee; A. Gahlmann; A. H. Zewail “Structural Dynamics of Free Amino Acids in Diffraction” Angewandte Chemie International Edition, 2012, 51, 99-102. 
  3. A. Gahlmann; I-R. Lee; A.H. Zewail “Direct Structural Determination of Conformations of Photoswitchable Molecules by Laser Desorption-Electron Diffraction” Angewandte Chemie International Edition 2010, 49, 6524-6527. 
  4. I-R. Lee; W. Lee; A. H. Zewail “Dynamics of Electrons in Ammonia Cages: The Discovery System of Solvation” Chemphyschem 2008, 9, 83-88.  
  5. I-R. Lee; L. Baares; A. H. Zewail “Direct Observation of the Primary Bond-twisting Dynamics of Stilbene Anion Radical” Journal of the American. Chemical. Society 2008, 130, 6708-6709. 
  6. H. Y. Chen; I-R. Lee; P. Y. Cheng ”Gas-phase Femtosecond Transient Absorption Spectroscopy” Review of Scientific. Instruments 2006, 77, 076105.  
  7. I-R. Lee; W. Lee; A. H. Zewail “The Primary Step of the Photoactive Yellow Protein: Isolated Chromophore Dynamics and Protein Directed Function” Proceedings of the National Academy of Sciences of the United States of America 2006, 103, 258-262. 
  8. D. H. Paik; I-R. Lee; D. S. Yang; J. S. Baskin; A. H. Zewail “Electrons in Finite-sized Water Cavities: Hydration Dynamics Observed in Real Time” Science 2004, 306, 672-675. 
  9. D. H. Paik; D. S. Yang; I-R. Lee; A. H. Zewail “The Transition State of Thermal Organic Reactions: Direct Observation in Real Time” Angewandte Chemie International Edition 2004, 43, 2830-2834.
  10. I-R. Lee; Y. C. Chung; W. K. Chen; X. P. Hong; P. Y. Cheng “Femtosecond Probing of Photodissociation Dynamics in Acyl Cyanides” Journal of Chemical Physics 2001, 115, 10656-10670.
  11. I-R. Lee; W. K. Chen; Y. C. Chung; P. Y. Cheng “A Direct Observation of Non-RRKM Behavior in Femtosecond Photophysically Activated Reactions” Journal of Physical Chemistry 2000, 104, 10595-10599.
  12. C. G. Lu; R. M. R. Hsieh; I-R. Lee; P. Y. Cheng “Ultrafast Dynamics of Gas-phase Excited-state Intramolecular Proton Transfer in 1-Hydroxy-2-Acetonaphthone” Chemical Physics Letter 1999, 310,103-110. 
年度 论文名称
2019 Mononuclear Manganese(III) Superoxo Complexes: Synthesis, Characterization, and Reactivity, Inorganic Chemistry, vol. 58, Jul. 2019
2019 Long-Range Hairpin Slippage Reconfiguration Dynamics in Trinucleotide Repeat Sequences, Journal of Physical Chemistry Letters, vol. 10, Jun. 2019
2018 Determination of Gold Ions in Human Urine Using Genetically Engineered Microorganisms on a Paper Device, ACS Sensors, vol. 3, Mar. 2018
2017 Parity-dependent hairpin configurations of repetitive DNA sequence promote slippage associated with DNA expansion, Proceedings of the National Academy of Sciences of the United States of America, vol. 114, Jul. 2017
I-R. Lee, T. Akinyi, T. Ha, “Single Molecule Observation of Direct Transfer of Escherichia Coli Single-Strand Binding Protein (SSB) between Single-Stranded DNA Molecules” Biophysical Society 57th Annual Meeting, February, 2013, Philadelphia, PA, USA. 
年度 论文名称
2019 Long-Range Slippery Hairpin Reconfiguration and its Mechanism in Trinucleotide Repeats Revealed by Single-Molecule Spectroscopy, Annual Meeting of the Biophysical Society, Mar. 02-06, 2019, Baltimore, USA
2015 The effect of magnesium cations on the folding of Gquadruplex, The 9th International Conference on Advanced Materials and Devices, Dec. 07-09, 2015, Jeju, Korea
2014 Single-molecule study on the formation and dynamics of G-quadruplex in the presence of magnesium cations, 19th East Asian Workshop on Chemical Dynamics, Oct. 04-07, 2014, Gold Coast, Australia
年度 计画名称 职称/担任之工作 计画期间 补助/委讬或合作机构
2018 单分子结构动态学研究-导致基因疾病的连续核酸序列 主持人 2016.04 ~ 2018.07 科技部(原国科会)
2017 以单分子光谱研究去氧核醣核酸分子之结构动态学 主持人 2014.08 ~ 2017.07 科技部(原国科会)
国家 学校名称 系所 学位 期间
美国 加州理工学院(Caltech) 化学系 博士 2003.06 ~ 2010.12
台湾 国立清华大学 化学所 硕士 2000.09 ~ 2002.06
台湾 国立清华大学 化学系 理学士 1995.09 ~ 1998.06
台湾 国立交通大学 应用化学系 1994.09 ~ 1995.06
服务机关名称 单位 职务 期间
美国伊利诺大学香槟分校(UIUC) 物理系 博士后研究员 2012.02 ~ 2014.01
美国加州理工学院(Caltech) 化学系 博士后研究员 2011.01 ~ 2012.01